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An amount of 2.5?��g of total RNA has been subjected to RT-PCR making use of Transcriptor Very first Strand cDNA Synthesis Package (Roche Diagnostics Company, Indianapolis, Within, United states of america) and anchored-oligo (dT)18 as well as haphazard hexamer primer, as well as the causing cDNA was made worse by PCR (Music et?al. 09). The actual Flag?+?HMOX1 gene segment was amplified because e
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