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But not Cul1 (Fig. 2B). These data indicate that the NH2-terminus of a Roc protein can direct specific Cullin binding. Chimeras between Roc1a and Roc2 behaved slightly differently than the ANBR and BNAR proteins. For instance, AN2R failed to bind any Cullin, even though it could be stably expressed (shown for Cul1 and Cul5 in Fig. 2C). There could be several reasons why AN2R fails to bind Cullin.
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